The total antioxidant activity, measured as percent inhibition of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was measured based on the method described by Alothman, Bhat, and
Karim (2009). Results were expressed as percentage inhibition
of DPPH radical, which was calculated using the following
equation:
DPPH radical scavenging activity %ðÞ¼ A0 –A1ðÞ
A 0
100
where A0 ¼ absorbance of the control, and A 1 ¼ absorbance of
sample extract.