The cooled sterilized solid substrate
(10 g) was placed in a 250ml conical flask, inoculated with 10%
of inoculum containing 2.6 × 106 conidia/ml, mixed uniformly
and incubated at 30 ◦C for 96h. Protease was extracted by suspending
the fermented substrate in distilled water (1:5) using
182 food and bioproducts processing 9 6 ( 2 0 1 5 ) 180–188
an orbital shaker at 30 ◦C, 150 rpm for 60min.