After thawing,
slices of 1 g of fruit were homogenized in methanol containing
1% (v/v) HCl and maintained at 4 C for 4 h. After
centrifugation at 10,000g, the absorbance of the supernatant
at 530 and 657 nm were recorded, and the anthocyanin
content was calculated as (A530–A657)/g of fresh weight. A
total of 12 strawberry fruits belonging to either transgenic
and control lines were analysed.
For drip loss analysis, 100 g of frozen strawberries were
weighed and let thaw at 23 C for 15 h. After this incubation
time, the drip was separated from the berries and the
drip loss estimated as the percentage of the original
weights. Three replicates per line were performed.