The trigger was set on the green fluorescence (520-nm) channel, and signals for total cell counting were collected on the combined 520-nm/630-nm(red fluorescence) dot plot.
For cellular biovolume estimations, additional signals were collected on the combined 520-nm/side scatter (SSC) dot plot.
An experimentally derived correlation factor was then used to determine the cellular
biovolume (15).
The quantification limit of the instrument was below 1,000 cells ml1 with an average standard deviation of less than 5% (14).