Tg and T
g of the inulin samples were investigated using a DSC
(DSC8230; Rigaku Co., Tokyo, Japan). In order to investigate the
effect of moisture content on Tg, the inulin samples held under
various RH conditions were prepared as mentioned above. Furthermore,
3–10% (w/w) aqueous solution was prepared in order
to investigate T
g of the inulin samples. An empty aluminum pan
was used as a reference, and the temperature and heat flow were
calibrated using indium and distilled water. The sample (2–10 mg)
was placed in an aluminum pan and hermetically sealed. In order to
evaluate anhydrous inulin Tg, inulin that had been vacuum-dried
at 60 ◦C for 12 h was put into the pan and held at 105 ◦C for 2 h