Protein bands were observed in proteins extracted from the untreated batter (lane 1) at the respective positions of the a and b subunits of glutelin (ca. 30 and 19 kDa, respectively), globulin (ca. 22 kDa), albumin (ca. 15 kDa), and prolamin (ca. 14 kDa) (Ju et al., 2001).