The resulting microcapsules were allowed to harden in the CaCl2 solution for 5, 10, 15, 20, 25 or 30 min. The oil-loaded alginate beads were collected from the cross-linking solution using a sieve. Finally, the mi- crobeads were rinsed twice with distilled water, tissue paper was used to absorb the surface excessive water and oil onto the wet microcapsules. Quantification of EOs loaded within AMSs was conducted by using the method described by Parris and his colleagues [15] with a slight modification, where the amount of EO enveloped into the microbeads was quantified by extracting the loaded oil from 0.5 g of these beads via their dissolution with 5 mL of sodium citrate (0.055 M) and 5 mL n-hexane. The absorbance was then measured at wavelength of 275 nm for thyme oil and at 280 nm for clove and cinnamon oils by using spectrophotometer model Ultrospec 2000 (Pharmacia Biotech Co. Cambridge, England). The amounts of EOs were calculated from plotted the standard curves for EOs with usage of dissolved alginate microbeads with no EO as a control.
Loading capacity (%)
The resulting microcapsules were allowed to harden in the CaCl2 solution for 5, 10, 15, 20, 25 or 30 min. The oil-loaded alginate beads were collected from the cross-linking solution using a sieve. Finally, the mi- crobeads were rinsed twice with distilled water, tissue paper was used to absorb the surface excessive water and oil onto the wet microcapsules. Quantification of EOs loaded within AMSs was conducted by using the method described by Parris and his colleagues [15] with a slight modification, where the amount of EO enveloped into the microbeads was quantified by extracting the loaded oil from 0.5 g of these beads via their dissolution with 5 mL of sodium citrate (0.055 M) and 5 mL n-hexane. The absorbance was then measured at wavelength of 275 nm for thyme oil and at 280 nm for clove and cinnamon oils by using spectrophotometer model Ultrospec 2000 (Pharmacia Biotech Co. Cambridge, England). The amounts of EOs were calculated from plotted the standard curves for EOs with usage of dissolved alginate microbeads with no EO as a control.Loading capacity (%)
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