The total phenolic content determination showed that P.alata leaf extract possessed higher phenolic content than
did P.edulis leaf extract (Table 1). When three different concentrations of each extract (0.1,1 and 10 lg/ml) were used in the TRAP assay to assess the antioxidant activity, we verified that both extracts presented significant antioxidant capacity in vitro only at final concentrations of 1 and 10 lg/ml. Furthermore, the antioxidant capacity (expressed as TEAC) was proportional to the amount of extract added and gave a linear response. As also shown in Table 1, the P. alata leaf extract showed higher antioxidant activity than did the P. edulis leaf extract. As depicted in Fig. 1, TEAC values of the extracts
and their total phenolic contents were well correlated for both P. alata (r = 0.997, p < 0.01) and P. edulis(r = 0.996, p < 0.01) leaf extracts, suggesting that the phenolic compounds make a major contribution to the antioxidant capacity of the extracts. Nevertheless, it is worth mentioning that the Folin–Ciocalteu phenol reagent gives only an approximate estimate of the total phenolic content.
The total phenolic content determination showed that P.alata leaf extract possessed higher phenolic content than
did P.edulis leaf extract (Table 1). When three different concentrations of each extract (0.1,1 and 10 lg/ml) were used in the TRAP assay to assess the antioxidant activity, we verified that both extracts presented significant antioxidant capacity in vitro only at final concentrations of 1 and 10 lg/ml. Furthermore, the antioxidant capacity (expressed as TEAC) was proportional to the amount of extract added and gave a linear response. As also shown in Table 1, the P. alata leaf extract showed higher antioxidant activity than did the P. edulis leaf extract. As depicted in Fig. 1, TEAC values of the extracts
and their total phenolic contents were well correlated for both P. alata (r = 0.997, p < 0.01) and P. edulis(r = 0.996, p < 0.01) leaf extracts, suggesting that the phenolic compounds make a major contribution to the antioxidant capacity of the extracts. Nevertheless, it is worth mentioning that the Folin–Ciocalteu phenol reagent gives only an approximate estimate of the total phenolic content.
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