Statistical analysis
The transformation efficiency recorded for transgenic plantlets regeneration after infection with Agrobacterium was based on values derived from the experiment replicated three times. To measure the variance in different repeated sets of experiment, standard deviation (SD) was calculated using the standard formula (easycalculation.com). One way ANOVA was used to compute the P-value to determine the statistical significance of transformation efficiency in replicated experiments using the R version 2.15.1 statistical computing software (r-project.org).