The samples selected after 0, 7, 14, 21, 28, and 35 days of storage
were immediately subjected to microbiological analysis, as
described by Kong, Wang, and Xiong (2007). On the day of analysis,
a 10 g surimi sample from each of the treatment was aseptically
removed. These samples were rinsed with 90 mL of 0.9% sterile
sodium chloride solution and homogenised in a stomacher for
1 min. Serial dilutions of the sample solution were mixed with agar
medium and then poured into petri dishes. The total plate counts,
which are expressed as CFU per gram of surimi, were determined
after incubation at 37 C for 48 h.
The samples selected after 0, 7, 14, 21, 28, and 35 days of storagewere immediately subjected to microbiological analysis, asdescribed by Kong, Wang, and Xiong (2007). On the day of analysis,a 10 g surimi sample from each of the treatment was asepticallyremoved. These samples were rinsed with 90 mL of 0.9% sterilesodium chloride solution and homogenised in a stomacher for1 min. Serial dilutions of the sample solution were mixed with agarmedium and then poured into petri dishes. The total plate counts,which are expressed as CFU per gram of surimi, were determinedafter incubation at 37 C for 48 h.
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