In the present study deals with the Oryza sativa .L was selected for callus induction and regeneration in in vitro
condition and also the physiological and biochemical studies were carried under the different salinity levels. The
different concentration of 2, 4-D were used to induce callus in the mature seeds and the callus was developed from
the region of the seeds and was visible within 7-10 days. The salt stress significantly influenced the germination
percentage was reduced gradually with increasing salt stress from 0 to 300 mM NaCl. The rate of proline
accumulation was observed much higher (205.4 mg/g) at 300 mM NaCl stress as compared to control (70.15). A
continuous decrease in protein content with increase in salt stress was observed, where around 64% reduction was
observed in plants under 300 mM NaCl stress as compared to the control plants. The starch content was higher in
control (617.2) than sample (345.5) with salinity stress. Among this study investigated that the overall showed better
tolerance to salt stress with a lesser extent of antagonistic effect of NaCl on germination and biomass production at
seedling stage.