Furthermore, the 18S rDNA was amplified by polymerase chain reaction (PCR) using primers designed to amplify 1500 bp fragment of the 18S rDNA region. The forward primer was 18SF149:50-GGAAGGG (G/A) TGTATTTATTAG -3 and the reverse primer was 18SR 701: 50-GTAAAAGTCCTGGTTCCC-30 After full identification, the fungus isolate was deposited in NRC culture collection under the name P. purpurogenum GE1. The fungal identification was carried out at City for Scientific Research, New Borg El-Arab City, Alexandria, Egypt.