Gels were then fixed in MeOH–HOAc–H2O (10:7:83 v/v) for 4 h and then stained with Sypro Ruby (Bio-Rad; Hercules, CA) overnight. After a brief destaining with ultrapure H2O, images were acquired using the Typhoon Trio (GE Healthcare; Piscataway, NJ, USA) with Sypro Ruby default settings.