Phenolic compounds in the corn cookies samples were extracted
according to the procedure described by Antoine, Peyron,
Lullien-Pellerin, Abecassis, and Rouau (2004). Total phenolics in
500 mg of samples were released by alkaline hydrolysis for 4 h at
room temperature using 10 mL of 4 mol/L NaOH. After the pH was
adjusted to 2.0 by 6 mol/L HCl, 5 mL of all the hydrolyzates were
extracted with 5 mL of ethyl acetate and diethyl ether (1:1, mL:mL)
for four times. FivemL of combined extracts were evaporated under
N2 stream at 30 C to dryness. Final residues were redissolved in
1.5 mL of methanol. Such prepared methanolic solutions were used
for the analyses of total phenolic compounds, flavonoids and
phenolic acids. The extracts were kept at 70 C prior to analyses.
All extractions were performed in duplicate per each replications of
baking experiment.
Phenolic compounds in the corn cookies samples were extractedaccording to the procedure described by Antoine, Peyron,Lullien-Pellerin, Abecassis, and Rouau (2004). Total phenolics in500 mg of samples were released by alkaline hydrolysis for 4 h atroom temperature using 10 mL of 4 mol/L NaOH. After the pH wasadjusted to 2.0 by 6 mol/L HCl, 5 mL of all the hydrolyzates wereextracted with 5 mL of ethyl acetate and diethyl ether (1:1, mL:mL)for four times. FivemL of combined extracts were evaporated underN2 stream at 30 C to dryness. Final residues were redissolved in1.5 mL of methanol. Such prepared methanolic solutions were usedfor the analyses of total phenolic compounds, flavonoids andphenolic acids. The extracts were kept at 70 C prior to analyses.All extractions were performed in duplicate per each replications ofbaking experiment.
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