4.3 Hydrolysis time variation ---Three samples were made by varying the autoclaving(hydrolysis) time of the solution. Three sample data are given in Table 03 Here we have got the highest percentage ethanol solution with the highest hydrolysis time. The same reason as like the Sulphuric acid concentration variation responsible here for the result. For a longer autoclaving time the hydrolysis process get more duration to occur. As a result the fermentable sugar concentration is increased in the solution which gives the higher percentage ethanol. Graphical presentation of those data is given in Figure 03
4.4 Fermentation time variation---This is done by varying the time of fermentation after the desired microorganism was added to the solution. The first sample was kept for three days fermentation, which is the standard fementation time. Another two samples was kept for five and seven days. Three sample data are given in Table 04. We got the highest cthanol percentage at the solution of seven days fermentation. This caused because of more fermentation time the microorganism gets more time to increase its concentration through the solution. As the concentration of microorganism is increased, so all of the fermentable sugar can convert to ethanol. As a whole ethanol percentage is increase, Graphical presentation of those data is given in Figure 04