Therefore, our objectives for the current effort are to (i) determine the chemical composition of Golden apple snails (GAS); (ii) establish the optimal conditions for the production of GAS protein hydrolysate produced from different combinations of independent parameters (temperature, ratio of enzyme substrate level, pH and hydrolysis time) on yield and antioxidant activity and iii) to compare the antioxidant activity of the optimized GAS hydrolysate with the commercial antioxidant. Kjeltec protein analyzer (Foss–Tecator AB, Sweden). Carbohydrate content was calculated by ‘difference’ method. Where 100% - (protein, fat, moisture and ash) %.