2.3. Acclimatization and Plant Regeneration
Thirty one in vitro generated plants with a well-developed shoot and root system were transferred to 8 cm dia.
plastic pots containing PRO-MIX (Premier Horticulture Inc., Quakertown, PA). Plants were acclimatized by
covering the pots with transparent plastic bags to maintain high relative humidity. After one week, bags were
removed and plants were treated with a water soluble 20-20-20 (N-P-K) fertilizer at biweekly intervals. Survival
percentage of plantlets was recorded. After four weeks of acclimation, plants were transferred to a greenhouse.