The chemical pesticide, imidacloprid (IMI) has long-lasting effectiveness against Hemiptera. IMI is commonly
used to control the brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae).
Some chemical pesticides, however, can induce the susceptibility of rice to BPH, which has indirectly
led to the resurgence of BPH. The mechanism of the chemical induction of the susceptibility of rice to
BPH was not previously understood. Here, a 44 K Agilent Rice Expression Microarray was used to identify
changes in gene expression that accompany IMI-induced rice susceptibility to BPH. The results showed
that 225 genes were differentially expressed, of which 117 were upregulated, and 108 were downregulated.
Gene ontology annotation and pathway analysis revealed that differentially expressed genes were
mainly classified into the eight functional groups: oxidation reduction, regulation of cellular process,
response to stress, electron carrier activity, metabolic process, transport, signal transducer, and organismal
development. The genes encoding plant lipid transfer protein, lignin peroxidase, and flavonol-
3-O-methyltransferenase may be important responses to the IMI-induced susceptibility of rice to BPH.
The reliability of the microarray data was verified by performing quantitative real-time PCR and the data
provide valuable information for further study of the molecular mechanism of IMI-induced susceptibility
of rice.
The chemical pesticide, imidacloprid (IMI) has long-lasting effectiveness against Hemiptera. IMI is commonlyused to control the brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae).Some chemical pesticides, however, can induce the susceptibility of rice to BPH, which has indirectlyled to the resurgence of BPH. The mechanism of the chemical induction of the susceptibility of rice toBPH was not previously understood. Here, a 44 K Agilent Rice Expression Microarray was used to identifychanges in gene expression that accompany IMI-induced rice susceptibility to BPH. The results showedthat 225 genes were differentially expressed, of which 117 were upregulated, and 108 were downregulated.Gene ontology annotation and pathway analysis revealed that differentially expressed genes weremainly classified into the eight functional groups: oxidation reduction, regulation of cellular process,response to stress, electron carrier activity, metabolic process, transport, signal transducer, and organismaldevelopment. The genes encoding plant lipid transfer protein, lignin peroxidase, and flavonol-3-O-methyltransferenase may be important responses to the IMI-induced susceptibility of rice to BPH.The reliability of the microarray data was verified by performing quantitative real-time PCR and the dataprovide valuable information for further study of the molecular mechanism of IMI-induced susceptibilityof rice.
การแปล กรุณารอสักครู่..