Freshly cut plant material from red clover wild type (Trifoliumpratense cv Milvus) and a low PPO mutant (M) lacking
expression of PPO4 were obtained from adjacentplots at Trawscoed Research Farm, Aberystwyth, which had been cut
and fertilised 4 weeks prior to harvesting. Forage was harvested from plots with a Haldrup 1500 plot harvester and cut to 5 cm above the soil to allow regrowth.