(c)Similar procedures of step 2 are performed with tetracycline, gyrase A and GFP primer sets and known control
templates.
(d)Perform a reaction of all primer sets (b-lactamase primers,
tetracycline, gyrase A, and GFP) with selected concentration (steps 2 and3) and known control template. Check
the size and band thickness of the products. Change ratio
of primer sets in reaction by increasing the amount of
primer in sets that show weak band signal or decrease
amount of primer sets that show very thick bands. Select
the primer concentrations that give similar yield of all
product sizes.