1. Introduction
Escherichia Coli (E. Coli) is part of the common microflora in the large intestine, causing no harm to the host. Because it is a fast growing prokaryote, it is widely used in laboratories for growing cultures for testing and experimentation. By identifying
the optimal growth conditions and environmental to lerances for the K-12 strain, E.coli may be more efficiently cultured for laboratory experimentation.