mple was commenced by raising the pH to 6.9 by the addition of
1 M NaHCO3/0.1 M NaHCO3. Then, 0.625 mL bile and pancreatic solution
(containing 0.225 g bile extract and 0.0375 g pancreatin in a
volumeof18.75mL0.1MNaHCO3)was addedfollowedby incubation
with shaking at 37 C for 2 h.