Measures of Metabolism
In the current study, transportation had effects on metabolism as demonstrated by changes (P < 0.05) in plasma concentrations of albumin, globulin, urea, total protein, and creative kinase relative to pert transportation (−24 h) values (Figure 1, Table 1). Albumin concentrations were reduced by 7% at 24 h (26.52 ± 0.33 g/L) compared with −24 h (28.26 ± 0.25 g/L; Figure 1A). Plasma globulin was decreased by 4.5 h and remained depressed by approximately 13% (41.36 ± 0.66 g/L vs. 47.04 ± 0.92 g/L at −24 h) throughout the time of blood collection (Figure 1B). Urea concentrations were not affected at 4.5 h but were decreased at 48 h (3.26 ± 0.16 nmol/L), 10% of the −24-h concentration (3.60 ± 0.18 nmol/L; Figure 1C). In concurrence, total plasma protein concentrations were decreased by 11% at 24 h (67.88 ± 0.61 g/L compared with 75.30 ± 0.92 g/L at −24 h; Figure 1D). In addition, a 39% decrease in plasma creative kinase was observed at 9.75 h (276.52 ± 31.02 U/L), followed by an increase of 221% at 24 h (1,229.72 ± 208.15 U/L compared with 554.63 ± 68.06 U/L at −24 h; Figure 1E). No changes in the concentration of plasma BHB, an energy substrate, were observed (Figure 1F).