according to Atmane et al. [29] in respect of myo-inositol,
thiamine, nicotinic acid and phytohormone content. The
shoots were incubated at 25 8C, in light at 50 mM m2 s
1
and 14 h photoperiod and transferred on to a fresh medium
every 3 months.
Agar (BactoAgar Difco 7 g l1
) was added to all the
media. The pH was adjusted to 5.75 prior to autoclaving.
Hormones were added to the media prior to sterilization,
which was carried out in an autoclave at 121 8C and under
increased pressure of 1 bar for 15 min.