After this, Genus Thinophilus, Nanothinophilus and Tepsimyia in Thailand will be used as out-groups for reconstructed phylogeny tree. Do you have any comment for this result?
Apart from universal primer COI, I am analyzing the sequences in 12S, 16S and also specific primer of COI but I have not finished yet.
I will send the result to you as soon as possible when I finish in this part.
Futuremore, For data analysis, Population genetics were analyzed using AMOVA and TCS program was used for Haplotype network. Phylogeny trees were reconstructed using DNAsp, Network and Beast program.