To maintain normal function, the pH of different cellular compartments, body fluids, and
organs must be tightly regulated by a process called acid-base homeostasis. The pH of
most commonly studied biological materials fall between 7 ~ 7.6. For example, the pH of
typical cytosol is 7.2, and mitochondrial matrix is 7.5. When investigating a biological
sample under an in vitro setting, one would want to keep the condition of the sample as
close as to where it’s derived from. The pH value of PBS is set to be within the range of
7 ~ 7.6, and often set to be 7.4 as the pH of blood nears 7.4, a value referred to as the
physiological pH in biology and medicine. In addition to maintaining a constant pH, PBS
in general has an osmolarity that matches those of the human body (isotonic) and is
non-toxic to the cells. As a result, PBS is frequently used as a washing buffer in tissue
culture and protein chemistry, or, when combined with BS
To maintain normal function, the pH of different cellular compartments, body fluids, and
organs must be tightly regulated by a process called acid-base homeostasis. The pH of
most commonly studied biological materials fall between 7 ~ 7.6. For example, the pH of
typical cytosol is 7.2, and mitochondrial matrix is 7.5. When investigating a biological
sample under an in vitro setting, one would want to keep the condition of the sample as
close as to where it’s derived from. The pH value of PBS is set to be within the range of
7 ~ 7.6, and often set to be 7.4 as the pH of blood nears 7.4, a value referred to as the
physiological pH in biology and medicine. In addition to maintaining a constant pH, PBS
in general has an osmolarity that matches those of the human body (isotonic) and is
non-toxic to the cells. As a result, PBS is frequently used as a washing buffer in tissue
culture and protein chemistry, or, when combined with BS
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