Preliminary, we optimized two systems: a homologous assay, which employed the anti-AFB1 antibody and the AFB1-HRP as the probe, and a heterologous assay, which employed the anti-AFM1 antibody and the same AFB1-HRP probe, since heterology is known to promote greater sensitivity (Holthues, Pfeifer-Fukumura, Sound, & Baumann, 2005) (Z.Wang et al., 2013).