One of the membrane phospholipids, phosphatidylserine
(PS), is normally located in the cytoplasmic surface of
the membrane. Once the cell undergoes apoptosis, the
PS molecules are translocated to the outer surface of the
cell. This translocation allows determination of PS, and
in turn apoptosis, when an agent such as Annexin-V labeled
with a florescent substance (for example, FITC) is
applied to the cell environment [11,12]. And then, using
a fluorescence microscopy allows to detect apoptotic
cells. However, one must be careful in the interpretation
of data, as a cell undergoing necrosis could also be
stained with annexin V+FITC, since the label may enter
in the cytoplasm through the damaged membrane
and stain the cytoplasm-exposed PS molecules, giving
a false positive result. If a fluorescence microcopy is not
available, then it is also possible to use annexin V-FITC
in a flow cytometry-based method [13].
One of the membrane phospholipids, phosphatidylserine
(PS), is normally located in the cytoplasmic surface of
the membrane. Once the cell undergoes apoptosis, the
PS molecules are translocated to the outer surface of the
cell. This translocation allows determination of PS, and
in turn apoptosis, when an agent such as Annexin-V labeled
with a florescent substance (for example, FITC) is
applied to the cell environment [11,12]. And then, using
a fluorescence microscopy allows to detect apoptotic
cells. However, one must be careful in the interpretation
of data, as a cell undergoing necrosis could also be
stained with annexin V+FITC, since the label may enter
in the cytoplasm through the damaged membrane
and stain the cytoplasm-exposed PS molecules, giving
a false positive result. If a fluorescence microcopy is not
available, then it is also possible to use annexin V-FITC
in a flow cytometry-based method [13].
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