The agreement to Rani and Nand was that the optimum C/N ratio for methane generation from ensilaged pineapple was at 20:1 [10]. Since nitrogen is an essential element for bacterial growth, the deficiency of nitrogen in the system of C/N ratio of 30:1 could inhibit growth of microorganism. On contrary, when nitrogen was excess in C/N ratio at 10:1, ammonia gas, which toxic to the microorganism, was produced and consequent to made total methane concentration to be decreased [11]. Nevertheless, this was not attentively