Each isolate was streaked at the middle of a Petri plate containing PDA medium and a mycelial disk (4 mm) from the peripheral region of 7 days old Botrytis cinerea’s culture was placed at approximately 2 cm from the bacteria. Plates were incubated at 25 C for 7 days and bioactivity evaluated by measuring the percentage of growth inhibition (Korsten et al. 1995) as indicated below:
GI% ¼ 100
R1R2 R1
R1 is the distance of fungal growth from the point of inoculation to the colony on control plates, and R2, the distance of fungal growth from the point of inoculation to the direction of the antagonist. Selected strains were identified using biochemical and molecular tools. Control plates were not inoculated with bacteria