Based on the findings of the study in Day 2 at a room temperature, the number of microorganisms in most of the Mu Yor sausage wrapped with chitosan film was found to be under the set criteria, while the number of microorganisms in Mu Yor sausage wrapped with plastic (PP) was found to be higher than the set criteria (Table 2). A possible explanation would be that the WVP value was low. In such conditions, facultative bacteria could grow well and caused spoil food. Mu Yor sausage wrapped with chitosan films without spice oil could be kept for 3 days which was longer than the study of Ministry of Public Health of Thailand (2007) which found that in such condition, Mu Yor sausage could be kept for 1 day.