pH 8. All these suspension were serially diluted up to
10-8. One hundred micro liters of appropriate dilutions was taken
and performed spread plate on different media viz. king’s B
medium (KBM), ashby’s mannitol agar (AMA), nitrogen free
medium(NFM), yeast extract mannitol agar (YMA),
azospirillum medium (AZ), pikovskaya medium (PM), bacillus
medium(BM). The plates prepared were incubated in room
temperature for 48 h. Bacterial cultures were maintained on the
respective slants and stored at 4°C for further use.