Aqueous leaf extract (ALE) of each crop were made by
crushing the leaves in sterile distilled water in a ratio of 1:1
w/v and passing through two layers of muslin cloth. The effect
of ALE on mycelial growth and sclerotial formation of S. rolfsii
was tested by poisoned food technique. For this 15 ml of
PDA medium amended with 5% ALE was poured in 90 mm
Petri dish. The Petri dishes were inoculated with 5 mm disc
excised from periphery of the actively growing 5-days old
culture of the isolate NRCG-SR-07. Three replications were
maintained for each, along with a control (without ALE). The
Petri dishes were incubated at 28±1°C in a BOD incubator.
The colony diameter was measured after 5 days by taking
two measurements at right angles. The number of sclerotia
per colony was counted after 20 days of incubation. Diameter
of 10 sclerotia was measured. The data from the three
replicated plates were averaged.