Figure 2. Nitric oxide (NO) synthesis in ABA inhibition of stomatal opening. (a)Epidermal strips were loaded with 10 mM
DAF2-DA for 15 min. After treatment with 0 mM of ABA, 10 mM of ABA or 10 mM of ABA + 100 mM cPTIO for 30 min, they were observed using an epifluorescence microscope. Data are shown as a ratio of blank (0 mM ABA )and treatment. Bars represent standard errors of means. (b,c) Images depicting NO synthesis induced by ABA treatment.(b) 0 mM ABA. (c) 10 mM ABA.