with plasma supernatant being collected and stored for future
analysis.
At each sampling point duplicate 20 μL blood samples were collected
into micropipettes for the determination of haemoglobin, and
triplicate 20 μL blood samples were collected into heparinised micro
haematocrit tubes for the determination of blood haematocrit concentration.
These data were used to estimate plasma volume and
to assess changes over time (Dill & Costill, 1974).