Ultra-high temperature (UHT) milk samples (n = 706) were obtained from markets in the central region of Croatia in the period February–May (winter-spring season), and May–July (summer season).
Samples were stored at 2–8 °C or frozen at −20 °C until further analysis of AFM1.
Milk samples were prepared according to the ELISA kit manufacturer's instructions. Samples were centrifuged for 10 min at 3500 × g at 10 °C. After centrifugation, the upper cream layer was completely removed by aspirating through a Pasteur pipette. Skimmed milk was used directly in the test (100 μl per well).