Total soluble solids and total acidity
The juice from 10 lemons for each replicate was used to deter-mine total soluble solids (TSS) and total acidity (TA). TSS were
determined with a digital refractometer Atago PR-101 (Atago Co.Ltd., Japan) at 20
C and results were the mean SE of 2 de-terminations for each sub-sample and expressed as
Brix (g/10 0 g).
The pH of the juice was recorded and then TA was determined by
potentiometric titration with 0.1 N NaOH up to pH 8.1, using 1 mL of
diluted juice in 25 mL distilled H2O and results were the
means SE of 2 determinations for each subsample and expressed
as g of citric acid equivalent per 10 0 g fresh weight.