Lysozyme lyophilized powder MW 14600 obtained from chicken
egg white (Himedia Cat. no. RM074) at a concentration of 1 mg/
ml was also added to the suspension. After 30 min on ice-bath,
the suspension was sonicated for 10 min at 40W amplitude and
8 s pulse. The sonicated sample was centrifuged at 10,000 rpm
for 30 min at 4 C. The soluble fraction of protein present in cleared
lysate was purified using Ni–NTA affinity chromatography.