Though brinjal exhibits maximum PPO activity, it is not used as source of enzyme in further studies. Brinjal is used as vegetable in daily in India therefore its use as enzyme source can increase the cost of process.Also it is not ethical to use food materials for getting enzyme preparation to treat wastewater. Banana peel is purely a waste agricultural product and readily available. Considering all these points,an extraction of PPO from banana peel is more advantageous than extraction from other sources. By realizing this,the present study was focused on banana peel PPO.