Fresh MRS broth and cell-free supernatant of LPS16 were
prepared as described above and concentrated to 3 mg/mL
by lyophilizer (Labconco, MO, USA). For LPS16 bacterial
cell, overnight culture LPS16 was washed with phosphatebuffered
saline and adjusted to OD600 Z 1.5. Six-mm paper
discs (Becton, Dickinson and Company) were soaked with 20
mL samples and dried for 5 minutes. H. pylori (McFarland
No. 2.0) was spread on BAH and discs were placed. Clear
zone sizes were measured after 2 days culture at 37C
under microaerophilic conditions.