It was also investigated whether RK affects the expression and
the secretion of adiponectin in differentiated 3T3-L1 adipocytes.
Four days of treatment of subconfluent cultures of 3T3-L1 preadipocytes
with 10 μM of RK increased markedly both the cellular
(l" Fig. 2a) and the secreted levels (l" Fig. 2b) of adiponectin. For
the immunoblot analysis of adiponectin expression levels, auraptenewas
used as a positive control [12]. Treatment with 10 μM of
RK led to an increase of 77 percent in the band intensity of adiponectin
compared to the negative control (l" Fig. 2a). As shown in
l" Fig. 2b, the secretion levels of adiponectin were significantly
upregulated by the RK treatment (10 μM)