was employed for analyzing b-carotene and lycopene in Gac oil samples. HPLC analysis was performed with an Agilent 1200 HPLC equipped with diode array detector system consisting of a Luna C18 (100 4.6 mm i.d.: 5 lm) direct-connect guard column coupled to a Jupiter C18 (250 4.6 mm i.d.: 5 lm) reversed phase column (Phenomenex).