The defatted rice bran was digested
with protease (60 C, 3 h), followed by digesting with amyloglucosidase
(60 C, 2 h) to remove protein and starch. Twenty volumes
of 4% NaOH were then added to extract hemicelluloses at room
temperature for 18 h under N2 flow, followed by filtration. The filtrate
was then neutralised with 5% acetic acid. After dialysis, four
volumes of 95% ethanol were then added to the filtrate. The precipitate
was oven-dried overnight (60 C, 16 h) in an air oven and then
weighed providing RBHB
The defatted rice bran was digestedwith protease (60 C, 3 h), followed by digesting with amyloglucosidase(60 C, 2 h) to remove protein and starch. Twenty volumesof 4% NaOH were then added to extract hemicelluloses at roomtemperature for 18 h under N2 flow, followed by filtration. The filtratewas then neutralised with 5% acetic acid. After dialysis, fourvolumes of 95% ethanol were then added to the filtrate. The precipitatewas oven-dried overnight (60 C, 16 h) in an air oven and thenweighed providing RBHB
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