Photosynthetic pigments from etiolated cucumber (Cucumis sativus var.
Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by
direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide
(DMF). The solvent is especially efficient when pigment concentration
is low; time and tools are saved and the loss of pigment that
usually occurs in more complicated extraction procedures is prevented.
The specific absorption coefficient of chlorophyll a in DMF was also
deternined