Carry out PCR with an initial denaturation step at 94 8C, 2 min; then, 30 cycles of denaturation (94 8C, 40 s), annealing (50 8C, 30 s) and elongation (72 8C, 1 and a half minutes) and a final step of elongation for 4 min.
Carry out PCR with an initial denaturation step at 94 8C, 2 min; then, 30 cycles of denaturation (94 8C, 40 s), annealing (50 8C,30 s) and elongation (72 8C, 1 and a half minutes) and a final step of elongation for 4 min.