Four bacterial strains of Bacillus spp. which were antagonistic to the mango anthracnose pathogen were isolated
and screened. Among them, TB09 and TB72 were identified by 16S rDNA sequence as Bacillus pumilus
and Bacillus thuringiensis, respectively. In vitro, the anthracnose fungus showed 88.87% and 80.07% of mycelia
growth inhibitions in presence of B. pumilus and B. thuringiensis, respectively and in vivo, the inhibitions
of the disease were 94.28% and 87.06%, respectively. Based on the Gas Chromatography–Mass Spectrometer
(GC–MS) analysis, 11 volatile compounds produced by B. pumilus and B. thuringiensis were identified.
Among them, five volatiles showed better inhibition effects on the pathogens. 2-nonanone, b-benzeneethanamine,
2-decanone completely inhibited mycelia growth in vitro at a concentration of 100 lL L1,
and thymol inhibited growth at concentrations of 50 mg L1 and 100 mg L1. The inhibition rate of
40 lL L1 artificial mixture of 5 volatiles was 98.75% in the plate test. The results showed that the two
screened antagonistic bacteria, and some of their produced volatiles and artificial mixtures could be promising
control methods for anthracnose in harvested mango fruit.
Four bacterial strains of Bacillus spp. which were antagonistic to the mango anthracnose pathogen were isolatedand screened. Among them, TB09 and TB72 were identified by 16S rDNA sequence as Bacillus pumilusand Bacillus thuringiensis, respectively. In vitro, the anthracnose fungus showed 88.87% and 80.07% of myceliagrowth inhibitions in presence of B. pumilus and B. thuringiensis, respectively and in vivo, the inhibitionsof the disease were 94.28% and 87.06%, respectively. Based on the Gas Chromatography–Mass Spectrometer(GC–MS) analysis, 11 volatile compounds produced by B. pumilus and B. thuringiensis were identified.Among them, five volatiles showed better inhibition effects on the pathogens. 2-nonanone, b-benzeneethanamine,2-decanone completely inhibited mycelia growth in vitro at a concentration of 100 lL L1,and thymol inhibited growth at concentrations of 50 mg L1 and 100 mg L1. The inhibition rate of40 lL L1 artificial mixture of 5 volatiles was 98.75% in the plate test. The results showed that the twoscreened antagonistic bacteria, and some of their produced volatiles and artificial mixtures could be promisingcontrol methods for anthracnose in harvested mango fruit.
การแปล กรุณารอสักครู่..
