Preparation of MHA
Suspend 38 gm of the medium in one liter of distilled water.
Heat with frequent agitation and boil for one minute to completely dissolve the medium.
Autoclave at 121°C for 15 minutes. Cool to room temperature.
Pour cooled Mueller Hinton Agar into sterile petri dishes on a level, horizontal surface to give uniform depth.
Allow to cool to room temperature.
Check for the final pH 7.3 ± 0.1 at 25ºC.
Store the plates at 2-8 ºC.