The solvents generally used for plant
dye extraction are ethanol, methanol,
acetone, chloroform, ether, clove oil, etc.
The dried material powder is weighed and
soaked in solvent in different percentages
and time durations. The crude dye extract
can be used for tissue staining after
extraction or can be further applied for
solvent evaporation in order to concentrate
the dye solution before staining.
Alternatively, the dried plant powder is
soaked in solvent to allow effective
percolation, then the soaked powder is
extracted in the solvent using Soxhlet
Extractor (Steam Heated Extractor). The
extract is then concentrated using rotary
evaporator and may further drying in the
drying oven (Okolie. 2008). Finally, the
extract is obtained in powdered form which
will be dissolved in the solvent or buffer at
the desire concentrations before tissue
staining.
Supercritical fluid extraction (SFE), an
alternative to conventional solvent
extraction for separation of organic
compound in many analytical processes as
well as extraction of plant natural dye has
gained wide acceptance in recent years
since this technique is safe for health,
inexpensive and harmonize with nature. SFE
uses carbon dioxide as a solvent. This
method is based on the enhanced solvating
power of gases above their critical point
(Samanta and Konar, 2011). Cardoni et al.
(2000) studied the extraction of lycopene
and β-carotene from ripe tomatoes using
SFE. The detail information and procedure
of this method has been reported by
Sapkale et al. (2010).
The solvents generally used for plantdye extraction are ethanol, methanol,acetone, chloroform, ether, clove oil, etc.The dried material powder is weighed andsoaked in solvent in different percentagesand time durations. The crude dye extractcan be used for tissue staining afterextraction or can be further applied forsolvent evaporation in order to concentratethe dye solution before staining.Alternatively, the dried plant powder issoaked in solvent to allow effectivepercolation, then the soaked powder isextracted in the solvent using SoxhletExtractor (Steam Heated Extractor). Theextract is then concentrated using rotaryevaporator and may further drying in thedrying oven (Okolie. 2008). Finally, theextract is obtained in powdered form whichwill be dissolved in the solvent or buffer atthe desire concentrations before tissuestaining.Supercritical fluid extraction (SFE), analternative to conventional solventextraction for separation of organiccompound in many analytical processes aswell as extraction of plant natural dye hasgained wide acceptance in recent yearssince this technique is safe for health,inexpensive and harmonize with nature. SFEuses carbon dioxide as a solvent. Thismethod is based on the enhanced solvatingpower of gases above their critical point(Samanta and Konar, 2011). Cardoni et al.(2000) studied the extraction of lycopeneand β-carotene from ripe tomatoes usingSFE. The detail information and procedureof this method has been reported by
Sapkale et al. (2010).
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