Anaerobic growth test
Basal medium [Peptone: 2 g; NaCl: 5 g; Agar: 0.3 g; KH2PO4: 0.3 g; bromothymol blue: (3 g) in 1 % aqueous solution, 5 ml] was prepared. An amount 0.5 ml 10% glucose suspension was added to each tube aseptically. For each isolate two test tubes were inoculated. One of the tubes was seal with paraffin, and incubated at 28 °C. An anaerobic growth was noticed if color change occurred from blue to yellow [9].