In this work, we discuss the possibilities of using bacterial cellulose (BC) produced by Gluconacetobacter
xylinus as carrier support for the immobilization of probiotic strains of Lactobacillus spp. In our study,
immobilization of the microorganisms was performed by the adsorption of bacterial cells on the surface
of the synthetized BC and by a simultaneous cultivation of the probiotic bacteria with cellulosesynthetizing
G. xylinus. Co-cultures were conducted in stationary cultures, in which BC was synthesized
as pellicles on the medium surface and in shaken cultures in which BC was synthetized in the forms
of beads. The experiments carried out also included the analysis of the survival of immobilized probiotic
bacteria in the presence of gastric juices and bile salts solution. The results showed that immobilization
of probiotic Lactobacillus in BC during co-culture with G. xylinus was the most effective immobilization
method, providing high-level protection of the microorganisms against the influence of gastric juices and
bile salts.